Análisis molecular de especies de la subfamilia Oestrinae (Diptera: Oestridae)
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2016
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Jaén: Universidad de Jaén
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[ES]Realizamos el estudio morfológico y molecular de especies de los géneros Cephenemya, Pharyngomyia y Oestrus parásitas de las cavidades nasofaríngeas causantes de miasis. Hemos estudiado los peritremos anteriores que constituyen un carácter morfológico adicional para diferenciar entre las especies de estos géneros. A pesar de haber diseñado primers en las regiones más variables del gen COI, los resultados de las PCR no permiten diferenciar las especies. Los resultados mediante la PCR-RFLP sugieren que mediante esta técnica puede ser factible identificar y diferenciar las distintas especies. La caracterización de los ITS de estas especies de oéstridos indica que son ricos en pares A-T, presentan un tamaño muy grande y son muy variables tanto en secuencia como en tamaño lo que hace que la reconstrucción filogenética con estos marcadores sea poco fiable. Finalmente, los análisis morfológico y moleculares de las larvas LII encontradas en el cerebro y meninges de los corzos indican que estas pertenecen a la especia C. stimulator.
[EN]We carry out the morphological and molecular study of species of the genera Cephenemyia, Pharyngomyia and Oestrus parasitic of nasopharyngeal cavities causing myiasis. We have studied the anterior peritremes and they constituted and additional morphological character to differentiate between species from these genera. Despite that primers were designed in the most variable regions of the COI gene, the PCR amplification results can not differentiate species. Analysis of PCR-RFLP suggests that this technique may be feasible to identify and differentiate the Oestridae species. Its characterization from oestrid species indicates that they are rich in A-T pairs, have a very large size, and are highly variable both in sequence and size which makes phylogenetic reconstruction with these markers unreliable. Finally, the morphological and molecular analysis of LII larvae found in the brain amd meninges of the roes indicate that belong to the species C. stimulator.
[EN]We carry out the morphological and molecular study of species of the genera Cephenemyia, Pharyngomyia and Oestrus parasitic of nasopharyngeal cavities causing myiasis. We have studied the anterior peritremes and they constituted and additional morphological character to differentiate between species from these genera. Despite that primers were designed in the most variable regions of the COI gene, the PCR amplification results can not differentiate species. Analysis of PCR-RFLP suggests that this technique may be feasible to identify and differentiate the Oestridae species. Its characterization from oestrid species indicates that they are rich in A-T pairs, have a very large size, and are highly variable both in sequence and size which makes phylogenetic reconstruction with these markers unreliable. Finally, the morphological and molecular analysis of LII larvae found in the brain amd meninges of the roes indicate that belong to the species C. stimulator.