Efecto del ayuno sobre los sistemas antioxidantes de hígado, cerebro, riñón y corazón de rata
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2016
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Jaén: Universidad de Jaén
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[ES]En las células hay dos enzimas antioxidantes llamadas glutatión reductasa (GR) y glutatión peroxidasa (GPx), que utilizan glutatión y NADPH. El objetivo de este trabajo ha sido determinar las características de este sistema en hígado, cerebro, riñón y corazón de rata y el efecto de un ayuno a largo plazo. Se determinó la actividad y expresión proteica de la GR y GPx y la concentración de metabolitos relacionados. La GR y la GPx muestran mayor actividad y expresión en riñón e hígado, respectivamente, además de haber mayor concentración de glutatión en hígado. El ayuno produce cambios en la GR, excepto en riñón, cambios en la GPx en todos los órganos y cambios de concentración de metabolitos. Esto demuestra que este sistema antioxidante no tiene la misma importancia en los distintos órganos frente al estrés oxidativo y que el ayuno afecta a este sistema dependiendo del órgano.
[EN]In the cells glutathione reductase (GR) and glutathione peroxidase (GPx) are two antioxidant enzymes that use glutathione and NADPH. The aims of this work have been to determine the biochemical properties of these systems in liver, brain, kidney and heart and the effect of a long-term starvation. Specific activity, protein expression of GR and GPx and the concentration of related metabolites were determined in all the organs. The GR and GPx show the highest activity and expression in kidney and liver, respectively, and the highest value of concentration of glutathione is shown in liver. Fasting produce changes in the GR, except in kidney, changes in the GPx in all the organs and changes of concentration of metabolites. This shows that this antioxidant system hasn't the same importance in the different organs against oxidative stress and fasting affect to this system dependin on the organ.
[EN]In the cells glutathione reductase (GR) and glutathione peroxidase (GPx) are two antioxidant enzymes that use glutathione and NADPH. The aims of this work have been to determine the biochemical properties of these systems in liver, brain, kidney and heart and the effect of a long-term starvation. Specific activity, protein expression of GR and GPx and the concentration of related metabolites were determined in all the organs. The GR and GPx show the highest activity and expression in kidney and liver, respectively, and the highest value of concentration of glutathione is shown in liver. Fasting produce changes in the GR, except in kidney, changes in the GPx in all the organs and changes of concentration of metabolites. This shows that this antioxidant system hasn't the same importance in the different organs against oxidative stress and fasting affect to this system dependin on the organ.