CULTIVO IN VITRO DE PLANTAS PARA LA OBTENCIÓN DE UNA COLECCIÓN DE CULTIVARES DE OLIVOS LIBRES DE PATÓGENOS.
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2020-10-19
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Jaén: Universidad de Jaén
Resumen
Olea europaea L., comúnmente conocido como olivo, es un árbol que se encuentra distribuido por toda la cuenca mediterránea. El objetivo general de este estudio fue la búsqueda de un protocolo de trabajo para la obtención de explantos de diferentes cultivares de olivo mediante cultivo in vitro y, por lo tanto, libres de patógenos, con la finalidad de crear un banco de germoplasma in vitro en la Universidad de Jaén. Por otra parte, este trabajo pretende hacer una revisión bibliográfica del uso de diferentes compuestos antifúngicos y antimicrobianos para el establecimiento de explantos libres de patógenos. En el proceso experimental llevado a cabo en este trabajo, el tejido vegetal utilizado fue, meristemos de olivo por su gran capacidad de división, aunque también se utilizaron segmentos nodales. Con ambos tejidos se comprobaron diferentes protocolos de desinfección y la utilización de diferentes medios de cultivo detallados en el trabajo.
Olea europaea L., commonly known as the olive tree, is a tree that is distributed throughout the Mediterranean basin. The general objective of this study was the search of a work protocol to obtain explants of different olive tree cultivars by means of in vitro culture and, therefore, free of pathogens, with the aim of creating an in vitro germplasm bank in the University of Jaén. On the other hand, this work aims to make a bibliographic review of the use of different antifungal and antimicrobial compounds for the establishment of pathogen-free explants. In the experimental process carried out in this work, the plant tissue used was, meristems of olive tree for its great capacity of division, although nodal segments were also used. With both tissues, different disinfection protocols and the use of different culture media detailed in the work were verified.
Olea europaea L., commonly known as the olive tree, is a tree that is distributed throughout the Mediterranean basin. The general objective of this study was the search of a work protocol to obtain explants of different olive tree cultivars by means of in vitro culture and, therefore, free of pathogens, with the aim of creating an in vitro germplasm bank in the University of Jaén. On the other hand, this work aims to make a bibliographic review of the use of different antifungal and antimicrobial compounds for the establishment of pathogen-free explants. In the experimental process carried out in this work, the plant tissue used was, meristems of olive tree for its great capacity of division, although nodal segments were also used. With both tissues, different disinfection protocols and the use of different culture media detailed in the work were verified.