Adición de compuestos bioactivos de hoja de olivo a aceites de oliva refinados.
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2021-07-09
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Jaén: Universidad de Jaén
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[ES] En el presente Trabajo Fin de Grado se ha llevado a cabo el enriquecimiento del aceite de oliva refinado con extracto fenólico previamente obtenido, por maceración, a partir de hoja de olivo (Olea europaea L.). Se han empleado dos muestras de hoja diferentes y se han realizado pruebas de adición de extracto diluido, concentrado y liofilizado, con y sin lecitina como emulsionante. Se ha determinado la capacidad antioxidante de los aceites y su contenido en compuestos fenólicos, por HPLC, para comprobar su estabilidad en el tiempo. Como conclusión se ha determinado que la mejor opción es la adición de extracto concentrado de hoja hasta una concentración de 2 g de extracto seco por litro de aceite refinado y una cantidad de lecitina, utilizada para mantener la emulsión, de 0,5 g/L aceite.
[EN] In this work, refined olive oil has been enriched with phenolic extract from olive leaf. The extraction of phenolic compounds was by a conventional extraction method called maceration. Two different leaf samples have been used for the enrichment of refined oil and tests have been carried out in the addition of diluted, concentrated and lyophilized extract, with and without lecithin as emulsifier. Finally, the content of phenolic compounds has been determined, as well as the antioxidant capacity to know the stability of the oils over time. The antioxidant capacity of the oils was determined by DPPH method and phenolic composition was analyzed by HPLC. In conclusion, the best option is the addition of concentrated leaf extract up to a concentration of 2 g of dry extract/L of refined oil and 0.5 g of lecithin/L of refined oil.
[EN] In this work, refined olive oil has been enriched with phenolic extract from olive leaf. The extraction of phenolic compounds was by a conventional extraction method called maceration. Two different leaf samples have been used for the enrichment of refined oil and tests have been carried out in the addition of diluted, concentrated and lyophilized extract, with and without lecithin as emulsifier. Finally, the content of phenolic compounds has been determined, as well as the antioxidant capacity to know the stability of the oils over time. The antioxidant capacity of the oils was determined by DPPH method and phenolic composition was analyzed by HPLC. In conclusion, the best option is the addition of concentrated leaf extract up to a concentration of 2 g of dry extract/L of refined oil and 0.5 g of lecithin/L of refined oil.