miR-200b durante la progresión in vitro en la especificación de células derivadas del epicardio
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2022-07-05
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Jaén: Universidad de Jaén
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[ES] El epitelio del epicardio constituye la capa más externa del corazón. Durante el desarrollo embrionario, las células epicárdicas cubren el miocardio, sufren un proceso de transición epitelio mesénquima (EMT) (E12.5-E15.5 en ratón) y migran a través de la pared miocárdica como EPDCs y se diferencian a células endoteliales y células musculares lisas que forman los vasos coronarios, así como fibroblastos cardiacos. Así mismo, la plasticidad celular que presenta el epicardio en la etapa embrionaria ha sido también observada en procesos patológicos cardiacos. Así, las EPDCs mediante EMT, pueden remodelar una región lesionada tras un infarto de miocardio. Todos estos procesos van a estar regulados por los microARN que desempeñan un papel fundamental en el control de la proliferación y maduración celular, como el miR-200b que influye en la regulación de la EMT. En este trabajo se investiga el papel del miR-200b en el tipo de divisiones celulares que experimentan las células epicárdicas y que modulan la EMT y la diferenciación de las EPDCs.
[EN] The epithelium of the epicardium constitutes the outermost layer of the heart. During embryonic development, epicardial cells cover the myocardium, undergo an epithelialmesenchymal transition (EMT) process (E12.5-E15.5 in mouse) and migrate through the myocardial wall as EPDCs and differentiate into endothelial cells and smooth muscle cells that form coronary vessels, as well as cardiac fibroblasts. Likewise, the cellular plasticity that the epicardium presents in the embryonic stage has also been observed in cardiac pathological processes. Thus, EPDCs by EMT can remodel an injured region after a myocardial infarction. All these processes will be regulated by microRNAs that play a fundamental role in the control of cell proliferation and maturation, such as miR-200b, which influences the regulation of EMT. In this work, the role of miR-200b in the type of cell divisions that epicardial cells undergo and that modulate EMT and EPDC differentiation is investigated.
[EN] The epithelium of the epicardium constitutes the outermost layer of the heart. During embryonic development, epicardial cells cover the myocardium, undergo an epithelialmesenchymal transition (EMT) process (E12.5-E15.5 in mouse) and migrate through the myocardial wall as EPDCs and differentiate into endothelial cells and smooth muscle cells that form coronary vessels, as well as cardiac fibroblasts. Likewise, the cellular plasticity that the epicardium presents in the embryonic stage has also been observed in cardiac pathological processes. Thus, EPDCs by EMT can remodel an injured region after a myocardial infarction. All these processes will be regulated by microRNAs that play a fundamental role in the control of cell proliferation and maturation, such as miR-200b, which influences the regulation of EMT. In this work, the role of miR-200b in the type of cell divisions that epicardial cells undergo and that modulate EMT and EPDC differentiation is investigated.