Análisis funcional de microRNAs en la diferenciación del linaje celular derivado del epicardio.
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2023-07-04
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Jaén: Universidad de Jaén
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[ES] El epicardio es la capa más externa que envuelve al corazón. Las células del mismo sufrirán una transición
epitelio mesénquima (EMT) invadiendo, de esta manera, el mesénquima subepicárdico o la pared del
miocardio dando lugar a células derivadas del epicardio (EPDC). Los principales reguladores de EMT son
la familia Snail y Prrx1 que también está relacionado con la misma y ambos cobran fuerza en este estudio.
Por otro lado, los factores de transcripción que se encargan de la regulación pre-transcripcional en el
desarrollo cardíaco son, entre otros, Nkx2.5, Gata4 y Srf. Este proceso también está regulado por los
microRNAs, que son pequeños fragmentos de RNA capaces de regular la expresión de RNA mensajero
influyendo de manera directa en su regulación post-transcripcional afectando a procesos posteriores. En
este proyecto se va a analizar cómo miR-106 y miR-130 afectan a los procesos de cardiogénesis y de EMT.
En los resultados se demuestra que miR-130 promueve EMT pero miR-106b no. También se demuestra
que la diferenciación cardiomiogénica viene dada por Gata4, Myh6 y Tnnt2 al sobreexpresar ambos
miRNAs.
[EN] The epicardium is the outermost layer that envelops the heart. The cells will undergo a mesenchymal epithelial transition (EMT) thus invading the subepicardial mesenchyma or myocardial wall resulting in cells derived from the epicardium (EPDC). The main regulators of EMT are the family Snail and Prrx1 which is also related to it and both gain strength in this study. On the other hand, transcription factors that are responsible for pre-transcriptional regulation in cardiac development are, among others, Nkx2.5, Gata4 and Srf. This process is also regulated by microRNAs, which are small fragments of RNA capable of regulating the expression of messenger RNA directly influencing its post-transcriptional regulation affecting subsequent processes. This project will analyze how miR-106 and miR-130 affect cardiogenesis and EMT processes. The results show that miR-130 promotes EMT but miR-106b does not. It is also shown that cardiomyogenic differentiation is given by Gata4, Myh6 and Tnnt2 by overexpressing both miRNAs.
[EN] The epicardium is the outermost layer that envelops the heart. The cells will undergo a mesenchymal epithelial transition (EMT) thus invading the subepicardial mesenchyma or myocardial wall resulting in cells derived from the epicardium (EPDC). The main regulators of EMT are the family Snail and Prrx1 which is also related to it and both gain strength in this study. On the other hand, transcription factors that are responsible for pre-transcriptional regulation in cardiac development are, among others, Nkx2.5, Gata4 and Srf. This process is also regulated by microRNAs, which are small fragments of RNA capable of regulating the expression of messenger RNA directly influencing its post-transcriptional regulation affecting subsequent processes. This project will analyze how miR-106 and miR-130 affect cardiogenesis and EMT processes. The results show that miR-130 promotes EMT but miR-106b does not. It is also shown that cardiomyogenic differentiation is given by Gata4, Myh6 and Tnnt2 by overexpressing both miRNAs.