Biosorción de iones plata y síntesis de nanopartículas
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2022-07-12
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Jaén: Universidad de Jaén
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[ES] Se estudió la capacidad para biosintetizar nanoparticulas de plata (Ag-NPs) de tres biomasas microbianas: el hongo Penicillium sp. 8L2, la levadura Rhodotorula mucilaginosa 1S1 y la biomasa fúngica residual de Botryosphaeria rhodina MAMB-05 (subtipo M3). Las Ag-NPs fueron identificadas por espectrofotometría ultravioleta visible en la región entre 400 nm y 430 nm, con un pico compatible con nanopartículas de AgCl (AgCl-NPs) entre 250 nm y 260 nm para la biomasa M3. Las imágenes de microscopía electrónica de transmisión analizadas con el software ImageJ, identificaron los diámetros mayoritarios para los tres casos. Finalmente se evaluó su efecto biocida calculando las concentraciones mínimas inhibitorias (CMIs) para 6 microorganismos, con valores entre 2,5 μg/mL y 256 μg/mL que en varios casos mejoraron los obtenidos para los antibióticos control. Todo ello indicó que la biosorción de Ag(I) podría tener como valor añadido la síntesis de Ag/AgCl-NPs con aplicaciones prometedoras en la biomedicina.
[EN] The ability to biosynthesize silver nanoparticles (Ag-NPs) of three microbial biomasses was studied: the fungus Penicillium sp. 8L2, the yeast Rhodotorula mucilaginosa 1S1 and the residual fungal biomass of Botryosphaeria rhodina MAMB-05 (subtype M3). Ag-NPs were identified by visible ultraviolet spectrophotometry in the region between 400 nm and 430 nm, with a peak compatible with AgCl nanoparticles (AgCl-NPs) between 250 nm and 260 nm for the M3 biomass. The transmission electron microscopy images analyzed with the ImageJ software identified the majority diameters for the three cases. Finally, its biocidal effect was evaluated by calculating the minimum inhibitory concentrations (MICs) for 6 microorganisms, with values between 2.5 μg/mL and 256 μg/mL, which in several cases improved those obtained for the control antibiotics. All this indicated that Ag(I) biosorption could have the added value of Ag/AgCl-NPs synthesis with promising applications in biomedicine.
[EN] The ability to biosynthesize silver nanoparticles (Ag-NPs) of three microbial biomasses was studied: the fungus Penicillium sp. 8L2, the yeast Rhodotorula mucilaginosa 1S1 and the residual fungal biomass of Botryosphaeria rhodina MAMB-05 (subtype M3). Ag-NPs were identified by visible ultraviolet spectrophotometry in the region between 400 nm and 430 nm, with a peak compatible with AgCl nanoparticles (AgCl-NPs) between 250 nm and 260 nm for the M3 biomass. The transmission electron microscopy images analyzed with the ImageJ software identified the majority diameters for the three cases. Finally, its biocidal effect was evaluated by calculating the minimum inhibitory concentrations (MICs) for 6 microorganisms, with values between 2.5 μg/mL and 256 μg/mL, which in several cases improved those obtained for the control antibiotics. All this indicated that Ag(I) biosorption could have the added value of Ag/AgCl-NPs synthesis with promising applications in biomedicine.