Resistencias de microorganismos cultivados en el medio Hicrome KPC
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2024-01-10
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Jaén: Universidad de Jaén
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En este estudio se ha trabajado para poner a punto la técnica para la utilización del medio de cultivo HiCrome KPC, un medio cromogénico y selectivo de bacterias Gram (-) productoras de Carbapenemasas. Las bacterias con las que se han realizado los ensayos proceden de alimentos vegetales, envasados o “a granel”. Además, se hacen ensayos para determinar la resistencia a distintos antibióticos y biocidas en las diferentes cepas bacterianas. Por último, se han identificado tres de las 31 cepas objeto de estudio mediante secuenciación de ADN genómico.
This study tried to fine-tune the proper technique for the use of HiCrome KPC culture medium, which is a chromogenic Gram (-) carbapenemase selective medium. The bacteria used in these tests came from plant which were bought packaged or "in bulk". In addition, tests were carried out in order to determine the resistance to various antibiotics and biocides in several bacterial strains. Finally, three of the 31 strains studied were identified by genomic DNA sequencing.
This study tried to fine-tune the proper technique for the use of HiCrome KPC culture medium, which is a chromogenic Gram (-) carbapenemase selective medium. The bacteria used in these tests came from plant which were bought packaged or "in bulk". In addition, tests were carried out in order to determine the resistance to various antibiotics and biocides in several bacterial strains. Finally, three of the 31 strains studied were identified by genomic DNA sequencing.