Adaptación celular a la inhibición de topoisomerasa II en células HeLa
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2016-10-27
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Jaén: Universidad de Jaén
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[ES] El “decatenation checkpoint” es un punto de control previo a la entrada en mitosis que controla el grado concatenaciones existentes entre los cromosomas. El enzima Topoisomerasa II es el encargado de eliminar dichas concatenaciones. El ICRF-193 es un inhibidor catalítico de esta enzima que permite activar artificialmente este punto de control. En este trabajo hemos evaluado la efectividad que tiene el ICRF-193 sobre el decatenation checkpoint. Usando como modelo células HeLa, se realizaron tratamientos simples o combinados con ICRF-193 y nocodazol a diferentes tiempos. Sobre estas muestras se hicieron análisis morfológicos y recuentos de células mitóticas para entender la dinámica de entrada y progresión de la mitosis. Los resultados sugieren que durante las primeras 12 horas el decatenation checkpoint es muy efectivo en su interrupción del ciclo celular. Pasado este tiempo, las células HeLa muestran adaptación a este punto de control y terminan entrando mayoritariamente en mitosis.
[EN] Decatenation checkpoint is a checkpoint previous to mitosis that controls concatenations between chromosomes. Topoisomerase II enzyme is responsible for the decatenation of these chromosomes. Bisdioxopiperazines, as ICRF-193, allow to artificially activate this checkpoint because it is a catalytic inhibitor of Topoisomerase II. We evaluated the prolonged effectiveness ICRF-193 has on time over the decatenation checkpoint. Using HeLa cells as a model, we did simple or combined treatments with ICRF-193 and nocodazole at different times. Each treatment were applied in 3 samples: 6, 12 and 24 hours. With a morphological analysis and cell counts in order to understand the dynamics of entry and progression of mitosis. The results suggest that during the first 12 hours, decatenation checkpoint is very effective in the cell cycle. After this time, HeLa cells show adaptation to this checkpoint and end up entry mostly in mitosis.
[EN] Decatenation checkpoint is a checkpoint previous to mitosis that controls concatenations between chromosomes. Topoisomerase II enzyme is responsible for the decatenation of these chromosomes. Bisdioxopiperazines, as ICRF-193, allow to artificially activate this checkpoint because it is a catalytic inhibitor of Topoisomerase II. We evaluated the prolonged effectiveness ICRF-193 has on time over the decatenation checkpoint. Using HeLa cells as a model, we did simple or combined treatments with ICRF-193 and nocodazole at different times. Each treatment were applied in 3 samples: 6, 12 and 24 hours. With a morphological analysis and cell counts in order to understand the dynamics of entry and progression of mitosis. The results suggest that during the first 12 hours, decatenation checkpoint is very effective in the cell cycle. After this time, HeLa cells show adaptation to this checkpoint and end up entry mostly in mitosis.
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