Utilización de un set de microsatélites para la tipificación y determinación de la huella alélica de cinco cultivares de olivo (Olea europaea subsp. europaea var. europaea)
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2018-12-04
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Jaén: Universidad de Jaén
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[ES] El olivo (Olea europaea) es una planta arbórea de hoja perenne. Olea europaea pertenece a la familia Oleaceae y al género Olea, el cual contiene unas 35 especies, y solo una de esas especies (Olea europea L.) la única con fruto comestible. Este estudio tiene como finalidad caracterizar 5 variedades de Olea europaea, utilizando 2 muestras de cada variedad. Para ello, se utiliza y analiza 12 marcadores SSR o microsatélites, y se obtiene así los perfiles alélicos de cada variedad. Se comprueba la coincidencia de una de las variedades del presente trabajo con otra variedad estudiada anteriormente en el laboratorio de Fisiología Vegetal. A su vez, se consigue ver la reproducibilidad del método, coincidiendo ambas muestras de la misma variedad en el perfil alélico. Y por último, se muestra el grado de polimorfismo de cada uno de los marcadores utilizados en este estudio.
[EN] The olive tree (Olea europaea) is a perennial plant. Olea europaea belongs to the Oleaceae family and the Olea genus, which contains about 35 species, and only one of those species (Olea europea L.) is the only one with edible fruit. The purpose of this study is to characterize 5 varieties of Olea europaea, using 2 samples of each variety. To do this, 12 SSR markers or microsatellites are used and analyzed, and thus the allelic profiles of each variety are obtained. lt is verified the coincidence of one of the varieties of the present work with another variety previously studied in the Plant Physiology laboratory. At the same time, we can see the reproducibility of the method, coinciding both samples ofthe same variety in the allelic profile. And finally, the degree of polymorphism of each of the markers used in this study is shown.
[EN] The olive tree (Olea europaea) is a perennial plant. Olea europaea belongs to the Oleaceae family and the Olea genus, which contains about 35 species, and only one of those species (Olea europea L.) is the only one with edible fruit. The purpose of this study is to characterize 5 varieties of Olea europaea, using 2 samples of each variety. To do this, 12 SSR markers or microsatellites are used and analyzed, and thus the allelic profiles of each variety are obtained. lt is verified the coincidence of one of the varieties of the present work with another variety previously studied in the Plant Physiology laboratory. At the same time, we can see the reproducibility of the method, coinciding both samples ofthe same variety in the allelic profile. And finally, the degree of polymorphism of each of the markers used in this study is shown.
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Fisiología vegetal