Bud27, una proteína tipo chaperona que participa en la transcripción en coordinación con las tres ARN polimerasas
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2016
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Jaén: Universidad de Jaén
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[ES]La transcripción en eucariotas se lleva a cabo por unas enzimas multiméricas denominadas ARN polimerasas. En la biogénesis de estas intervienen varios factores de ensamblaje, entre los que encontramos los complejos de prefoldina, asociados a la prefoldina-like Bud27 en levaduras. Tanto Bud27, como su ortólogo en humanos, URI, median diversos procesos celulares esenciales. Con este trabajo, se han identificado genes supresores del fenotipo de termosensibilidad de una cepa de Saccharomyces cerevisiae, mutante para el gen de esta prefoldina, bud27Δ. A través de análisis bioinformáticos, se ha elaborado una red de interacción de BUD27 con los genes más probables de ejercer la supresión, para visualizar globalmente la conexión de BUD27 con los procesos en los que dichos genes están implicados. Bud27, además, es la primera proteína demostrada que interacciona físicamente con las tres ARN polimerasas y participa en la biogénesis de las mismas, en un proceso dependiente de Rpb5, subunidad común a las tres ARN polimerasas. Con la utilización de una cepa mutante del dominio amino-terminal de Rpb5 se ha querido profundizar en la conexión entre Bud27 y Rpb5 en el ensamblaje de las ARN polimerasas. Los resultados muestran que en la cepa mutante existen defectos de ensamblaje y acumulación citoplasmática de las ARN polimerasas. Futuros estudios con los diferentes supresores permitirán establecer la conexión entre estos y BUD27, así como determinar de manera más precisa la relación entre Bud27 y Rpb5.
[EN]Transcription in eukaryote is carried out by multimeric enzymes, the RNA polymerases. Several assembly factors participate in the biogenesis of these complexes, among them the prefoldin complexes associated to the prefoldin-like Bud27 in yeast. Bud27 and its orthologue in humans, URI, mediate essential cellular processes. In this research work, we have identified genes acting as suppressors of the thermosensitive phenotype of the S. cerevisiae bud27Δ mutant strain. Using bioinformatic tools we have performed an interaction network between BUD27 and the most probable genes to exert the supression, in order to visualise the connection between BUD27 and those genes that are the most likely candidate to exert the suppression, so as to visualize the relationship between BUD27 and the processes where they participate. In addition, Bud27 is the first example of a protein that contacts the three eukaryotic RNA pols and participates in the biogenesis of the enzymes, in an Rpb5-dependent manner. By using an RPB5 mutant lacking part of the amino-terminal domain of Rpb5, we wanted to go more insight into the connection between Bud27 and Rpb5 in RNA pols assembly. The results show defects in RNA pols assembly and their cytoplasmic accumulation. New studies with the different suppressors will allow us to establish the connection between them and BUD27, as well as to go more insight into the relationship between Bud27 and Rpb5.
[EN]Transcription in eukaryote is carried out by multimeric enzymes, the RNA polymerases. Several assembly factors participate in the biogenesis of these complexes, among them the prefoldin complexes associated to the prefoldin-like Bud27 in yeast. Bud27 and its orthologue in humans, URI, mediate essential cellular processes. In this research work, we have identified genes acting as suppressors of the thermosensitive phenotype of the S. cerevisiae bud27Δ mutant strain. Using bioinformatic tools we have performed an interaction network between BUD27 and the most probable genes to exert the supression, in order to visualise the connection between BUD27 and those genes that are the most likely candidate to exert the suppression, so as to visualize the relationship between BUD27 and the processes where they participate. In addition, Bud27 is the first example of a protein that contacts the three eukaryotic RNA pols and participates in the biogenesis of the enzymes, in an Rpb5-dependent manner. By using an RPB5 mutant lacking part of the amino-terminal domain of Rpb5, we wanted to go more insight into the connection between Bud27 and Rpb5 in RNA pols assembly. The results show defects in RNA pols assembly and their cytoplasmic accumulation. New studies with the different suppressors will allow us to establish the connection between them and BUD27, as well as to go more insight into the relationship between Bud27 and Rpb5.