Modulación de la diferenciación celular del proepicardio mediado por microRNAs
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2017-10-31
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Jaén: Universidad de Jaén
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[ES]El proepicardio, en cultivos ex vivo, posee la capacidad de formar cardiomiocitos, células en las cuales no genera por sí mismo. Esta cualidad permite al epicardio adulto desarrollado generar cardiomiocitos tras un proceso cardíaco dañino mediante la administración inicial de Timosina 134 {Tl34}1.
Los microRNAs son una clase de pequeñas moléculas de RNA con una longitud entre 19 y 22 nucleótidos, de cadena sencilla y no codificantes que actúan como reguladores postranscripcionales, fundamentalmente inhibiendo la expresión génica2• Actualmente se sabe que los microRNAs desempeñan un papel crucial en la regulación de la expresión génica controlando diversas vías de proliferación y diferenciación celulares3• Hemos observado el papel funcional de distintos microRNAs {miR 27A, miR 278, miR 100, miR 125 y miR 195) en el proceso de diferenciación celular del proepicardio de pollo. Para ello analizamos si distintas condiciones de cultivo del proepicardio son capaces de incrementar o de disminuir la expresión de estos microRNAs en el contexto del desarrollo del proepicardio.
[EN]Proepicardium, in ex vivo cultures, has the capacity to form cardiomyocytes, cells in which it does not generate by itself. This quality allows the developed adult epicardium to generate cardiocytes after a harmful cardiac process by the initial administration of Thymosin 134 (Tl34)1• MicroRNAs are a class of small RNA molecules with a length between 19 and 22 nucleotides, singlestranded and non-coding that act as post-transcriptional regulators, primarily inhibiting gene expression2• MicroRNA is now known to play a crucial role in the regulation of gene expression that controls various pathways of cellular proliferation and differentiation3• We have observed the functional role of different microRNAs (miR 27A, miR 278, miR 100, miR 125 and miR 195) in the process of cell differentiation of the chicken process. In order to do so, the different conditions of culture of the process are analyzed in their capacity to increase or decrease the expression of these microRNAs in the context of the development of the procedure.
[EN]Proepicardium, in ex vivo cultures, has the capacity to form cardiomyocytes, cells in which it does not generate by itself. This quality allows the developed adult epicardium to generate cardiocytes after a harmful cardiac process by the initial administration of Thymosin 134 (Tl34)1• MicroRNAs are a class of small RNA molecules with a length between 19 and 22 nucleotides, singlestranded and non-coding that act as post-transcriptional regulators, primarily inhibiting gene expression2• MicroRNA is now known to play a crucial role in the regulation of gene expression that controls various pathways of cellular proliferation and differentiation3• We have observed the functional role of different microRNAs (miR 27A, miR 278, miR 100, miR 125 and miR 195) in the process of cell differentiation of the chicken process. In order to do so, the different conditions of culture of the process are analyzed in their capacity to increase or decrease the expression of these microRNAs in the context of the development of the procedure.