Caracterización de la variación natural en Sinapis alba (Brassicaceae L.) en la resistencia a herbicidas de preemergencia
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2024-09-05
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[ES] Sinapis alba es una especie ruderal ampliamente distribuida que representa una amenaza para los cultivos más importantes de la cuenca mediterránea sobre todo si presenta resistencia al herbicida de preemergencia oxifluorfen, que inhibe la enzima PPO (protoporfirinógeno oxidasa). Para estudiar la variación natural de la resistencia a este herbicida en S. alba se han realizado una serie de experimentos en tres fases. En la fase de screening se obtuvo el porcentaje de germinación para plantas control y plantas tratadas con oxifluorfen; en la fase de ensayos moleculares se secuenciaron individuos resistentes y se realizaron alineamientos del gen PPX2 frente a individuos sensibles para describir las mutaciones presentes; y en la última fase se realizaron estudios bioinformáticos de asociación de genes (GWAS) relacionados con la resistencia al oxifluorfen y análisis estadísticos. Los resultados confirman que el oxifluorfen tiene un efecto negativo sobre la germinación de las semillas, además el gen PPX2 tiene mutaciones que podrían estar relacionadas con esta resistencia.
[EN] Sinapis alba is a widely distributed weed that represents a threat to the most important crops in the Mediterranean basin, especially if it is resistant to the pre-emergence herbicide oxyfluorfen, which inhibits the PPO (protoporphyrinogen oxidase) enzyme. A series of experiments were carried out in three phases to study the natural variation of resistance to this herbicide in S. alba. In the screening phase, the germination percentage was obtained for control and oxyfluorfen-treated plants; in the molecular assay phase, resistant individuals were sequenced and PPX2 gene alignments were performed against sensitive individuals to describe the mutations present; and in the last phase, bioinformatic gene association studies (GWAS) related to oxyfluorfen resistance and statistical analyses were performed. The results confirm that oxyfluorfen harms seed germination; moreover, the PPX2 gene has mutations that could be related to this resistance.
[EN] Sinapis alba is a widely distributed weed that represents a threat to the most important crops in the Mediterranean basin, especially if it is resistant to the pre-emergence herbicide oxyfluorfen, which inhibits the PPO (protoporphyrinogen oxidase) enzyme. A series of experiments were carried out in three phases to study the natural variation of resistance to this herbicide in S. alba. In the screening phase, the germination percentage was obtained for control and oxyfluorfen-treated plants; in the molecular assay phase, resistant individuals were sequenced and PPX2 gene alignments were performed against sensitive individuals to describe the mutations present; and in the last phase, bioinformatic gene association studies (GWAS) related to oxyfluorfen resistance and statistical analyses were performed. The results confirm that oxyfluorfen harms seed germination; moreover, the PPX2 gene has mutations that could be related to this resistance.