Efecto de la gonadotropina coriónica humana en la meiosis de machos de la especie Xenopus tropicalis
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2019-07-09
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Jaén: Universidad de Jaén
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[ES] La gonadotropiria coriónica humana (hCG) se utiliza para el apareamiento in vivo de Xenopus tropicalis por su efecto en ambos sexos. Por un lado, hace que la hembra ovule, mientras que por el otro hace que el macho se abrace a la hembra (amplexo), facilitándose de esta manera la reproducción. El objetivo de este trabajo es investigar si la inyección de hCG en machos tiene algún efecto beneficioso cuando se realizan cruzamientos in vitro. Para ello, se han evaluado la concentración espermática, el número de células en las diferentes fases de la meiosis y la histología testicular en machos tratados con hCG (durante 5 o 18 horas) y se han comparado con los mismos parámetros en machos no tratados. La exposición a hCG durante 5 horas aumenta la concentración espermática en los machos. Sin embargo, no se observan diferencias en los tratados durante 18 horas. Con estos resultados podemos concluir que la exposición a hCG durante 5 horas presenta beneficios relativos a la concentración espermática. Cuando se analizan las etapas de la meiosis o la histología testicular, no se observan diferencias entre las muestras procedentes de machos no tratados y las de machos tratados (independientemente del tiempo). Teniendo en cuenta estos resultados se recomienda la inyección de gonadotropina coriónica humana para la realización de los cruzamientos in vitro debido al aumento en la concentración espermática que produce.
[EN] Human chorionic gonadotropin (hCG) is used for in vivo mating of Xenopus tropicalis because of its effect on both sexes. On the one hand it causes the female to ovulate, while on the other it causes the male to embrace the female (amplexus), thus facilitating reproduction. The objective of this work is to investigate if the injection of hCG in males has any beneficia! effect when crossing in vitro. For this purpose, the sperm concentration, the number of cells in the different phases of meiosis and testicular histology in males treated with hCG (for 5 or 18 hours) have been evaluated and compared with the same parameters in untreated males. Exposure to hCG for 5 hours increases sperm concentration in males. However, no differences were observed in those treated for 18 hours. With these results we can conclude that the exposure to hCG during 5 hours presents benefits relative to the sperm concentration. When analysing the stages of meiosis or testicular histology, no differences are observed between samples from untreated males and treated males (regardless of time). Taking into account these results, the injection of human chorionic gonadotropin is recommended for in vitro crossings dueto the increase in sperm concentration produced.
[EN] Human chorionic gonadotropin (hCG) is used for in vivo mating of Xenopus tropicalis because of its effect on both sexes. On the one hand it causes the female to ovulate, while on the other it causes the male to embrace the female (amplexus), thus facilitating reproduction. The objective of this work is to investigate if the injection of hCG in males has any beneficia! effect when crossing in vitro. For this purpose, the sperm concentration, the number of cells in the different phases of meiosis and testicular histology in males treated with hCG (for 5 or 18 hours) have been evaluated and compared with the same parameters in untreated males. Exposure to hCG for 5 hours increases sperm concentration in males. However, no differences were observed in those treated for 18 hours. With these results we can conclude that the exposure to hCG during 5 hours presents benefits relative to the sperm concentration. When analysing the stages of meiosis or testicular histology, no differences are observed between samples from untreated males and treated males (regardless of time). Taking into account these results, the injection of human chorionic gonadotropin is recommended for in vitro crossings dueto the increase in sperm concentration produced.