Efecto del tratamiento crónico con hormona tiroidea sobre la liberación de exosomas en orina de rata
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2016-03-04
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Jaén: Universidad de Jaén
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[ES] Se sabe que el hipertiroidismo se acompaña de alteraciones renales que ocasionan proteinuria. En el presente trabajo se pretende comprobar si en el hipertiroidismo existen alteraciones en la excreción urinaria de exosomas y en la actividad enzimática urinaria de aminopeptidasas. Para ello se ha comprobado la variabilidad de la cantidad de exosomas en muestras de orina de ratas control y ratas tratadas con tiroxina. Los exosomas se aislaron mediante dos centrifugaciones sucesivas a 17.000 y 200.000 g, y se cuantificaron determinando la proteína total presente. También se analizó la actividad enzimática presente en cada fracción urinaria por fluorimetría cinética. Las ratas hipertiroideas excretaron mayor cantidad de exosomas que el grupo control. Sin embargo, no se observaron diferencias en las actividades aminopeptidásicas urinarias. Los resultados obtenidos podrían ser de gran interés para analizar el papel que juegan los exosomas urinarios como fuente de biomarcadores para el diagnóstico de diferentes patologías renales.
[EN] It is known that hyperthyroidism is associated with renal alterations which evoke proteinuria. The aim of the present work is to demonstrate if hyperthyroidism is accompanied with alterations in the urinary excretion of exosomes and alterations of urinary aminopeptidase activities. Variability of exosomes excretion in urine samples from control and thyroxine-treated rats has been studied. Exosomes were isolated by two subsequent centrifugations at 17.000 and 200.000 g, and they were quantified through analyzing total protein. Besides, enzymatic activity of each urinary fraction was measured by kinetic fluorimetry. Hyperthyroid rats excreted higher levels of exosomes than control group. Nevertheless, there were not any differences in urinary aminopeptidasic activities. These results could be of great interest to analyze the role of urinary exosomes as a source of biomarkers for the diagnosis of renal diseases.
[EN] It is known that hyperthyroidism is associated with renal alterations which evoke proteinuria. The aim of the present work is to demonstrate if hyperthyroidism is accompanied with alterations in the urinary excretion of exosomes and alterations of urinary aminopeptidase activities. Variability of exosomes excretion in urine samples from control and thyroxine-treated rats has been studied. Exosomes were isolated by two subsequent centrifugations at 17.000 and 200.000 g, and they were quantified through analyzing total protein. Besides, enzymatic activity of each urinary fraction was measured by kinetic fluorimetry. Hyperthyroid rats excreted higher levels of exosomes than control group. Nevertheless, there were not any differences in urinary aminopeptidasic activities. These results could be of great interest to analyze the role of urinary exosomes as a source of biomarkers for the diagnosis of renal diseases.