DETERMINACIÓN DE HORMONAS VEGETALES MEDIANTE HPLC-MASAS TRIPLE CUADRUPOLO EN DIFERENTES LÍNEAS GENÉTICAS DE BRACHYPODIUM DISTACHYON SOMETIDAS A ESTRÉS POR SEQUÍA.
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2020-10-19
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Jaén: Universidad de Jaén
Resumen
Cuantificación de siete fitohormonas (ácido salicílico, ácido metilsalicílico, ácido abscísico, giberelinas, ácido jasmónico, ácido metiljasmónico y Trans-zeatina) en ocho líneas genéticas del género Brachypodium distachyon mediante la técnica HPLC- espectometría de masas de triple cuadrupolo que han estado sometidas a un periodo de 10 días en ausencia total de agua para comprobar el comportamiento hormonal respecto a las plantas control que no se sometieron a sequía.
La respuesta hormonal fue diversa, observando fluctuaciones en las hormonas que presentan una mayor implicación frente a un estrés por déficit hídrico (ácido salicílico, su derivado el ácido metilsalicílico, ácido abscísico, ácido jasmónico y su derivado ácido metiljasmónico).
Los genotipos que presentaron mayor sensibilidad a la sequía fueron DSNSCA 5 y Bd21, mientras
Quantification of seven phytohormones (salicylic acid, methylsalicylic acid, abscisic acid, gibberellins, jasmonic acid, methyljasmonic acid and Trans-zeatin) in eight genetic lines of the genus Brachypodium distachyon using the HPLC-triple quadrupole mass spectrometry technique, plants that have been subdued to a period of 10 days in the total absence of wáter to check the hormonal behavior with respect to the control plants that were not subdued to drought. The hormonal response was diverse, observing fluctuations in the hormones that have a greatest implication against water stress (salicylic acid, its derivative methyl salicylic acid, abscisic acid, jasmonic acid and its derivative methyljasmonic acid). The genotypes with the highest sensitivity to drought were DSNSCA 5 and Bd21, while the JCIM and GRAB 16 were the genetic lines that had the most resistance to this stress.
Quantification of seven phytohormones (salicylic acid, methylsalicylic acid, abscisic acid, gibberellins, jasmonic acid, methyljasmonic acid and Trans-zeatin) in eight genetic lines of the genus Brachypodium distachyon using the HPLC-triple quadrupole mass spectrometry technique, plants that have been subdued to a period of 10 days in the total absence of wáter to check the hormonal behavior with respect to the control plants that were not subdued to drought. The hormonal response was diverse, observing fluctuations in the hormones that have a greatest implication against water stress (salicylic acid, its derivative methyl salicylic acid, abscisic acid, jasmonic acid and its derivative methyljasmonic acid). The genotypes with the highest sensitivity to drought were DSNSCA 5 and Bd21, while the JCIM and GRAB 16 were the genetic lines that had the most resistance to this stress.