GENERACIÓN DE UNA CEPA DE SACCHAROMYCES CEREVISIAE PORTANDO UN ALELO DEL GEN BUD27 QUE EXPRESARÁ UNA PROTEÍNA SENSIBLE A DEGRADACIÓN POR AUXINAS
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2020-10-13
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Jaén: Universidad de Jaén
Resumen
Las plantas poseen un sistema que en presencia de la hormona vegetal auxina (Ácido indol-3-acético, IAA o ácidol-naftalenacético, NAA) degrada rápida y eficazmente una proteína diana. Este sistema es conocido como sistema degron inducible por auxinas (AID) en el que participa la ubiquitina ligasa SCF E3 y el proteosoma. Otros eucariotas no tienen sistema respuesta a las auxinas, pero sí comparten la vía de degradación SCF con las plantas, pudiéndose utilizar el sistema degron en células no vegetales. En este trabajo se pretende obtener una cepa de Saccharomyces cerevisiae que porte en un alelo del gen BUD27 que permita expresar la proteína Bud27 fusionada al sistema AID para poder degradar la proteína Bud27 y analizar así su funcionalidad y efectos fenotípicos.
Ali plants do have a system that in the presence of the vegetal hermane auxin (indole-3-acetic acid, IAA or 1-naphthalenacetic acid, NAA) rapidly and efficiently deplete into a target protein. This system is known as the auxin-inducible degron system (AID) in which the ubiquitin ligase SCF E3 and the proteasome participate. Other eukaryotes do not have a response system to auxins, but they do share the SCF degradation way with plants. lt seems interesting to remark that the degron system may be used in non-vegetal cells. The main aim of this work is to obtain a strain of Saccharomyces cerevisiae carrying a BUD27 allele, expressin a Bud27 protein fused to the AID system in arder to degrade Bud27, in arder to analyze its functionality and phenotypical effects.
Ali plants do have a system that in the presence of the vegetal hermane auxin (indole-3-acetic acid, IAA or 1-naphthalenacetic acid, NAA) rapidly and efficiently deplete into a target protein. This system is known as the auxin-inducible degron system (AID) in which the ubiquitin ligase SCF E3 and the proteasome participate. Other eukaryotes do not have a response system to auxins, but they do share the SCF degradation way with plants. lt seems interesting to remark that the degron system may be used in non-vegetal cells. The main aim of this work is to obtain a strain of Saccharomyces cerevisiae carrying a BUD27 allele, expressin a Bud27 protein fused to the AID system in arder to degrade Bud27, in arder to analyze its functionality and phenotypical effects.