Obtención de marcadores genéticos del tamaño de fruto en el olivo cultivado
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2019-06-12
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Jaén: Universidad de Jaén
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[ES]El tamaño del fruto en el olivo cultivado es consistentemente de mayor tamaño que en los olivos silvestres. Sin embargo, resulta difícil para los mejoradores conseguir variedades que aumenten el tamaño del mismo. La obtención de marcadores genéticos que puedan usarse en programas de mejora para este carácter sería muy interesante. Por este motivo en este trabajo se ha realizado un análisis mediante GWAS de posibles marcadores y genes asociados a la determinación del tamaño de fruto a partir de 51 genotipos, 41 correspondientes a olivos cultivados y 10 a olivos silvestres. Este análisis dio como resultado la identificación de 113 marcadores genéticos correspondientes a 39 genes que mostraron una muy alta significación estadística con la determinación del tamaño del fruto. El genotipado de un par de genes en una población segregante de un cruzamiento de Picual x Frantoio produjo resultados consistentes con los resultados encontrados en el GWAS.
[EN] The size of the fruit in the cultivated olive trees is consistently larger than in wild olives. However, it is difficult for breeders to obtain varieties that increase the size of this trait. Obtaining genetic markers to be used in breeding programs for this trait would be most interesting. For this reason, in this work a GWAS analysis of genetic markers and genes associated to the determination of the fruit size was made from 51 genotypes, 41 corresponding to cultivated olive trees and 10 to wild olives. As a result, 113 genetic markers corresponding to 39 genes that showed a very high statistical significance with the determination of the size of the fruit were identified. The genotyping of a pair of genes in a segregating population of a Picual x Frantoio cross, produced consistent results with the GWAS analysis.
[EN] The size of the fruit in the cultivated olive trees is consistently larger than in wild olives. However, it is difficult for breeders to obtain varieties that increase the size of this trait. Obtaining genetic markers to be used in breeding programs for this trait would be most interesting. For this reason, in this work a GWAS analysis of genetic markers and genes associated to the determination of the fruit size was made from 51 genotypes, 41 corresponding to cultivated olive trees and 10 to wild olives. As a result, 113 genetic markers corresponding to 39 genes that showed a very high statistical significance with the determination of the size of the fruit were identified. The genotyping of a pair of genes in a segregating population of a Picual x Frantoio cross, produced consistent results with the GWAS analysis.