EFECTO DE LA INGESTA ELEVADA DE FRUCTOSA SOBRE LAS TOXINAS URÉMICAS DE ORIGEN INTESTINAL.
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2020-10-19
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Jaén: Universidad de Jaén
Resumen
La ingesta elevada de fructosa supone un gran peligro para la composición de la microbiota intestinal que cuando se ve alterada produce efectos perjudiciales sobre la salud humana. Uno de ellos es el aumento de toxinas urémicas de origen intestinal, en nuestro caso estudiamos el incremento de indoxyl sulfato en muestras de plasma en ratas. Para ello realizamos la determinación de dicha toxina mediante cromatografía liquida de alta resolución (HPLC) con detección de fluorescencia y realizamos un análisis entre las muestras control y las muestras sometidas a una ingesta elevada de fructosa, no encontrando diferencias significativas. Asimismo, analizamos los niveles de creatinina en plasma por el método de Jaffé con el autoanalizador Spin 120 ya que la ingesta de fructosa también produce disfunción renal, sin embargo, tampoco encontramos diferencias significativas entre los niveles de fructosa y creatinina. Para finalizar nuestro estudio, llevamos a cabo la correlación entre el IS y la creatinina para entender los efectos del indoxyl sulfato en la función renal. A pesar de no tener correlación ni diferencias significativas, debemos de cuidar nuestra microbiota intestinal mediante la alimentación.
The high intake of fructose poses a great danger to the composition of the gut microbiota, which, when altered, produces harmful effects on human health. One of them is the increase in uremic toxins of intestinal origin, in our case we studied the increase of indoxyl sulfate in plasma samples in rats. For this purpose, we performed the determination of this toxin by high-performance liquid chromatography (HPLC) with fluorescence detection, and we performed an analysis between the control samples and the samples subjected to a high intake of fructose, not finding significant differences. Likewise, we analyzed plasma creatinine levels by the Jaffé method with the Spin 120 autoanalyzer since fructose intake also produces renal dysfunction, however, we also found no significant differences fructose and creatinine levels. To conclude our study, we performed the correlation between IS and creatinine to understand the effects of indoxyl sulfate on renal function. Despite not having any correlation or significant differences, we must take care of our gut microbiota by feeding.
The high intake of fructose poses a great danger to the composition of the gut microbiota, which, when altered, produces harmful effects on human health. One of them is the increase in uremic toxins of intestinal origin, in our case we studied the increase of indoxyl sulfate in plasma samples in rats. For this purpose, we performed the determination of this toxin by high-performance liquid chromatography (HPLC) with fluorescence detection, and we performed an analysis between the control samples and the samples subjected to a high intake of fructose, not finding significant differences. Likewise, we analyzed plasma creatinine levels by the Jaffé method with the Spin 120 autoanalyzer since fructose intake also produces renal dysfunction, however, we also found no significant differences fructose and creatinine levels. To conclude our study, we performed the correlation between IS and creatinine to understand the effects of indoxyl sulfate on renal function. Despite not having any correlation or significant differences, we must take care of our gut microbiota by feeding.