Caracterización proteómica y estudio comparativo de dos cepas de enterococos
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2024-01-10
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Jaén: Universidad de Jaén
Resumen
Con el paso del tiempo, el tipo de dieta ha ido variando y hoy en día los
alimentos procesados han desplazado el consumo de frutas y vegetales. Cada
vez se publican más estudios que muestran como esos cambios en el patrón
dietético repercuten directamente sobre nuestra salud al afectar, por ejemplo, a
la microbiota gastrointestinal. En investigaciones anteriores a este trabajo, se
quiso ver como afectaba el tipo de grasa de la dieta a la microbiota
gastrointestinal de diferentes grupos de ratones y se asoció una secuencia 16S
de una cepa de E. faecalis, con un aumento de la presión arterial en un grupo de
ratones alimentados con una dieta rica en mantequilla. Esta cepa fue
caracterizada y se obtuvo su proteoma mediante espectrometría de masas. En
este trabajo de fin de máster se lleva a cabo un estudio comparativo con el
proteoma de otra cepa aislada en el mismo experimento y que no presentó
correlación alguna. Con ello se quiere investigar si alguna de las proteínas
obtenidas a partir de esta cepa podría ser la responsable de la subida de la
presión arterial en ese grupo de ratones.
Over time, the type of diet has been changing and nowadays processed food has displaced the consumption of fruits and vegetables. More and more studies are being published showing how these changes in the dietary pattern have a direct impact on our health by affecting, for example, the gastrointestinal microbiota. In investigations previous to this study, they wanted to see how the type of dietary fat affected the gastrointestinal microbiota of different groups of mice and a 16S sequence of a strain of E. faecalis was associated with an increase in blood pressure in a group of mice fed a diet rich in butter. This strain was characterized and its proteome was obtained by mass spectrometry. In this end-of-master project, a comparative study is carried out with the proteome of another strain isolated in the same experiment and which did not present any correlation. The aim is to investigate if any of the proteins obtained from this strain could be responsible for the rise in blood pressure in this group of mice
Over time, the type of diet has been changing and nowadays processed food has displaced the consumption of fruits and vegetables. More and more studies are being published showing how these changes in the dietary pattern have a direct impact on our health by affecting, for example, the gastrointestinal microbiota. In investigations previous to this study, they wanted to see how the type of dietary fat affected the gastrointestinal microbiota of different groups of mice and a 16S sequence of a strain of E. faecalis was associated with an increase in blood pressure in a group of mice fed a diet rich in butter. This strain was characterized and its proteome was obtained by mass spectrometry. In this end-of-master project, a comparative study is carried out with the proteome of another strain isolated in the same experiment and which did not present any correlation. The aim is to investigate if any of the proteins obtained from this strain could be responsible for the rise in blood pressure in this group of mice