MÉTODOS DE DETECCIÓN Y ELIMINACIÓN DE SALMONELLA SPP EN VEGETALES DE HOJA
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2022-03-11
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Jaén: Universidad de Jaén
Resumen
[ES] El objetivo del presente trabajo fue realizar una búsqueda bibliográfica acerca de la presencia del
género Salmonella en vegetales de hoja, así como los métodos de detección del patógeno y las diferentes técnicas de reducción. Para ello, en primer lugar, se hizo una búsqueda sobre este patógeno y sus características, así como su incidencia en el mundo y, en concreto se buscó en los vegetales de hoja. Se pudo comprobar como con los métodos de desinfección con químicos como peroxiacético, ozono, productos a base de cloro, etc, se consiguieron reducciones muy significativas de Salmonella en alimentos variados como lechuga, lombarda, acelga..., al igual que la desinfección con bacteriófagos. Por último, se realizó una búsqueda acerca de los métodos de detección de Salmonella, considerando el más idóneo el uso de PCR para detectar el gen de virulencia del patógeno, llegando incluso a necesitarse una PCR-RT para mejorar su detección.
[EN] The objective of the present work was to carry out a bibliographic search about the presence of the genus Salmonella in leafy vegetables, as well as the methods for detecting the pathogen and the different reduction techniques. To do this, in the first place, a search was made on this pathogen and its characteristics, as well as its incidence in the world and, specifically, leaf vegetables were searched. It was possible to verify that with the disinfection methods with chemicals such as peroxyacetic, ozone, chlorine-based products, etc., very significant reductions of Salmonella were achieved in various foods such as lettuce, red cabbage, chard ..., as well as disinfection with bacteriophages. Finally, a search was carried out on the methods of detection of Salmonella, considering the most suitable the use of PCR to detect the virulence gene of the pathogen, even requiring an RT-PCR to improve its detection.
[EN] The objective of the present work was to carry out a bibliographic search about the presence of the genus Salmonella in leafy vegetables, as well as the methods for detecting the pathogen and the different reduction techniques. To do this, in the first place, a search was made on this pathogen and its characteristics, as well as its incidence in the world and, specifically, leaf vegetables were searched. It was possible to verify that with the disinfection methods with chemicals such as peroxyacetic, ozone, chlorine-based products, etc., very significant reductions of Salmonella were achieved in various foods such as lettuce, red cabbage, chard ..., as well as disinfection with bacteriophages. Finally, a search was carried out on the methods of detection of Salmonella, considering the most suitable the use of PCR to detect the virulence gene of the pathogen, even requiring an RT-PCR to improve its detection.