Efectos del eugenol en invertebrados acuáticos: Evaluación sobre Daphnia magna
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2022-07-05
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Jaén: Universidad de Jaén
Resumen
[ES] La esencia de clavo es una sustancia natural, que se utiliza como anestésico en vertebrados, que se degradaen el medio, pero aún no hay muchos estudios que hayan mostrado los efectos y las aplicaciones del eugenol, en animales invertebrados. En este estudio se pretende analizar los efectos del eugenol en
Daphnia magna, y su potencialidad como anestésico. Se han realizado 3 pruebas para evaluar los efectos
de esta especie en eugenol, una para evaluar el comportamiento, otra para ver el efecto en reproducción
y la última para ver el efecto en frecuencia cardíaca. La concentración de 120mg/L produce un efecto
anestésico, que modifica el comportamiento y que permite recuperar a los individuos con rapidez una vez cesa la exposición. En exposición crónica (21 días) de eugenol a concentraciones de 0,2, 1 y 2 mg/L no hay efecto en la reproducción de D. magna. Hay una disminución de la frecuencia cardíaca tras la exposición a eugenol a la máxima concentración tanto en machos y hembras.
[EN] Clove oil is a natural substance used as anesthetic in vertebrates, which is easily degraded in the environment, but there is a lack of information with invertebrate animals. This study aims to analyze the effects of eugenol on Daphnia magna, and its potential as an anesthetic. Three tests have been carried out to evaluate the impact of clove oil in this specie on behavior, reproduction and heart rate. It has been observed that under 120mg/L eugenol an anesthetic effect is produced, which modifies behavior and allows individuals to recover quickly once exposure ceases. It has also been found that in a chronic exposure (21 days) to eugenol at concentrations of 0,2, 1 and 2 mg/L there is no toxic effect on the reproduction of D.magna. Additionally, there was a decrease in heart rate, in males and females of D. magna, after exposure to eugenol at the maximum concentration tested.
[EN] Clove oil is a natural substance used as anesthetic in vertebrates, which is easily degraded in the environment, but there is a lack of information with invertebrate animals. This study aims to analyze the effects of eugenol on Daphnia magna, and its potential as an anesthetic. Three tests have been carried out to evaluate the impact of clove oil in this specie on behavior, reproduction and heart rate. It has been observed that under 120mg/L eugenol an anesthetic effect is produced, which modifies behavior and allows individuals to recover quickly once exposure ceases. It has also been found that in a chronic exposure (21 days) to eugenol at concentrations of 0,2, 1 and 2 mg/L there is no toxic effect on the reproduction of D.magna. Additionally, there was a decrease in heart rate, in males and females of D. magna, after exposure to eugenol at the maximum concentration tested.