DESARROLLO Y VALIDACIÓN DE UN MÉTODO PARA DETERMINACIÓN DE METALES PESADOS EN POLEN.
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2020-10-15
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Jaén: Universidad de Jaén
Resumen
La identificación de metales pesados como contaminantes atmosféricos suscita gran interés desde mediados del
siglo XX, siendo su bio-monitorización un medio muy eficaz para la detección de contaminación. Por ello, se ha
desarrollado y validado un método para cuantificar 20 metales pesados en polen, mediante espectrometría de
masas con plasma de acoplamiento inductivo (ICP-MS) tras digestión por microondas. Se han evaluado los
parámetros de desempeño del método, encontrándose que los intervalos lineales de trabajo varían de 0,1 a 1000 μg
L-1, con límites de detección y cuantificación en los rangos de 0,012 a 6,2 ng g-1 y 0,039 a 21 ng g-1 respectivamente.
La dispersión observada en términos de repetibilidad fue inferior al 8,5%. El sesgo se evaluó con un material de
referencia certificado, obteniéndose desviaciones menores de 9,6%. Asimismo, se realizó un estudio de la
concentración de estos metales en polen de cinco especies vegetales (Pistacia lentiscus, Pistacia terebinthus, Phoenix
canariensis, Pópulus alba y Pinus halepensis). Ello permitió hacer una aproximación a la eficiencia del polen como
bioindicador de contaminación ambiental, hallándose diferencias significativas en el contenido de Ag, Al, Ba, Ni, Pb,
Ti y Zn.
Since the middle of the 20th century, the identification of heavy metals as atmospheric pollutants has been of great interest, being their bio-monitoring a very effective tool in pollution detection. Therefore, a new method has been developed and validated for the quantification of 20 heavy metals in pollen by inductively coupled plasma mass spectrometry (ICP-MS) after microwave digestion. Method performance characteristics have been assessed, finding out that linear working ranges vary from 0,1 to 1000 μg L-1, with limits of detection and quantification in ranges from 0,012 to 6,2 ng g-1 and 0,039 to 21 ng g-1 respectively. The observed dispersion was below 8,5% in terms of repeatability. Bias was evaluated with a certified reference material, achieving differences below 9,6%. Furthermore, a study of the concentration of these metals in pollen of five vegetal species (Pistacia lentiscus, Pistacia terebinthus, Phoenix canariensis, Pópulus alba and Pinus halepensis) was carried out. This enabled an approximation about pollen efficiency as a bioindicator of air pollution, finding out significant differences in Ag, Al, Ba, Ni, Pb, Ti and Zn content.
Since the middle of the 20th century, the identification of heavy metals as atmospheric pollutants has been of great interest, being their bio-monitoring a very effective tool in pollution detection. Therefore, a new method has been developed and validated for the quantification of 20 heavy metals in pollen by inductively coupled plasma mass spectrometry (ICP-MS) after microwave digestion. Method performance characteristics have been assessed, finding out that linear working ranges vary from 0,1 to 1000 μg L-1, with limits of detection and quantification in ranges from 0,012 to 6,2 ng g-1 and 0,039 to 21 ng g-1 respectively. The observed dispersion was below 8,5% in terms of repeatability. Bias was evaluated with a certified reference material, achieving differences below 9,6%. Furthermore, a study of the concentration of these metals in pollen of five vegetal species (Pistacia lentiscus, Pistacia terebinthus, Phoenix canariensis, Pópulus alba and Pinus halepensis) was carried out. This enabled an approximation about pollen efficiency as a bioindicator of air pollution, finding out significant differences in Ag, Al, Ba, Ni, Pb, Ti and Zn content.