Purificación de Peroxirredoxinas a partir de órganos de animales
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2019-07-13
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Jaén: Universidad de Jaén
Resumen
Se realiza la purificación de la peroxirredoxina Prdx6 de ratón mediante técnicas cromatográficas convencionales con el fin de analizar sus características catalíticas. Prdx6 pertenece al grupo de peroxirredoxinas 1-Cys, de las cuales no se ha llegado a un absoluto consenso sobre el mecanismo catalítico. Se trata de probar si la peroxirredoxina Prdx6 purificada de pulmón de ratón se ajusta al mecanismo catalítico demostrado con la peroxirredoxina 1-Cys (Prxlp) propia de Saccharomyces cerevisiae.
A method of purification of the mouse peroxiredoxin Prdx6 has been performed by conventional chromatographic techniques in order to analyze its catalytic characteristics. Prdx6 belongs to the group of peroxiredoxins 1-Cys, of which no consensus on the catalytic mechanism has been reached. The aim is to test whether peroxidase Prdx6 purified from mouse lung conforms to the catalytic mechanism demonstrated with the 1-Cys peroxiredoxin (Prxlp) characteristic of Saccharomyces cerevisiae.
A method of purification of the mouse peroxiredoxin Prdx6 has been performed by conventional chromatographic techniques in order to analyze its catalytic characteristics. Prdx6 belongs to the group of peroxiredoxins 1-Cys, of which no consensus on the catalytic mechanism has been reached. The aim is to test whether peroxidase Prdx6 purified from mouse lung conforms to the catalytic mechanism demonstrated with the 1-Cys peroxiredoxin (Prxlp) characteristic of Saccharomyces cerevisiae.