CARACTERIZACIÓN DE MICROSATÉLITES EN DOS ESPECIES DE OÉSTRIDOS
Archivos
NO SE HA AUTORIZADO la consulta de los documentos asociados
Fecha
2019-07-03
Autores
Título de la revista
ISSN de la revista
Título del volumen
Editor
Jaén: Universidad de Jaén
Resumen
[ES]Oestridae es una familia de dípteros parásitos causantes de miasis, entre los que se encuentran Cephenemyia stimulator y Oestrus ovis, parásitos del corzo y ovinos, respectivamente. En este estudio, se analizaron las secuencias de ambas especies para obtener 15 microsatélites, para los que se diseñaron primers específicos que fueron utilizados para caracterizar genotípicamente poblaciones de C. stimulator y O. ovis. Se describieron un total de 54 alelos distintos para C. stimulator, mientras que en el caso de O. ovis se observó una mayor variabilidad, con un total de 113 alelos, habiéndose calculado también la frecuencia de cada uno de ellos. Por otro lado, en C. stimulator se obtuvo un valor medio de heterocigosidad observada de 0,44, siendo para O. ovis de 0,48. Además, mientras que las poblaciones de C. stimulator mostraban estar en el equilibrio de Hardy-Weinberg, se encontraron desviaciones en O. ovis, probablemente a causa de la presencia de alelos nulos. El estudio de estos microsatélites será muy útil para investigar muchos aspectos de estas especies como estructura poblacional, dispersión y dinámica de poblaciones.
Palabras clave: Cephenemyia stimulator, Oestrus ovis, microsatélites.
[EN]Oestridae is a family of dipterous parasites that cause myiasis, including Cephenemyia stimulator and Oestrus ovis, parasites of roe deer and sheep, respectively. In this study, the sequences of both species were analyzed in order to obtain 15 microsatellites, which have been used to genotypically characterize C. stimulator and O. ovis populations. A total of 54 alleles were described for C. stimulator, whereas O. ovis showed a greater variability with 113 alleles in total. Frequencies were also calculated for each allele. Apart from that, C. stimulator showed a mean observed heterozygosity value of 0,44, being 0,48 for O. ovis. Furthermore, while the populations of C. stimulator were in Hardy-Weinberg equilibrium, deviations were found in the case of O. ovis, probably due to the presence of null alleles. Studying these microsatellites is very useful to investigate other aspects of these species such as population structure, dispersion and population dynamics. Key words: Cephenemyia stimulator, Oestrus ovis, microsatellite markers.
[EN]Oestridae is a family of dipterous parasites that cause myiasis, including Cephenemyia stimulator and Oestrus ovis, parasites of roe deer and sheep, respectively. In this study, the sequences of both species were analyzed in order to obtain 15 microsatellites, which have been used to genotypically characterize C. stimulator and O. ovis populations. A total of 54 alleles were described for C. stimulator, whereas O. ovis showed a greater variability with 113 alleles in total. Frequencies were also calculated for each allele. Apart from that, C. stimulator showed a mean observed heterozygosity value of 0,44, being 0,48 for O. ovis. Furthermore, while the populations of C. stimulator were in Hardy-Weinberg equilibrium, deviations were found in the case of O. ovis, probably due to the presence of null alleles. Studying these microsatellites is very useful to investigate other aspects of these species such as population structure, dispersion and population dynamics. Key words: Cephenemyia stimulator, Oestrus ovis, microsatellite markers.