Caracterización de marcadores moleculares de la especie Cephenemyia stimulator de la subfamilia Oestrinae (Diptera, Oestridae)
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2018-11-29
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Jaén: Universidad de Jaén
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[ES] En este trabajo estudia molecularmente la especie C. stimulator de la familia de dípteros Oestridae, que provoca miasis en el corzo. Hemos amplificado mediante PCR, clonado y secuenciado los marcadores moleculares COI, 28s y ITS-1 e ITS-2 de varios individuos procedentes de poblaciones francesas y españolas. En nuestro trabajo describimos por primera vez los marcadores moleculares 28S, ITS-1 e ITS-2, y amplían los datos sobre el COI de la especie C. stimulator. Hemos demostrado que presentan porcentajes de identidad muy altos. Presentan variaciones debidas a mutaciones por cambio de bases, deleciones e inserciones, en ocasiones relacionadas con microsatélites. Los ITS-1 y el ITS-2 son los marcadores que presentan mayores rangos de variación en los porcentajes de identidad, y presentan tamaños superiores a los descritos en otras especies de dípteros. En conjunto los resultados indican que no hay diferencias entre los individuos de las poblaciones española y francesa estudiadas.
[EN] In this work we perform molecular studies of the species C. stimulator from the family Oestridae (Diptera), which causes myiasis in roe deer. We have amplified by PCR, cloned and sequenced the molecular markers COI, 28s and ITS-1 and ITS-2 in several individuals from French and Spanish populations. We described for the first time the molecular markers 28S, ITS-1 and ITS-2, and expand the data about COI of the species C. stimulator. We have shown that these markers present very high percentages of identity. Although, they present variations due to base changes, deletions and insertions mutations, sometimes related to microsatellites. ITS-1 and the ITS-2 are the markers that have greater ranges of variation in the percentages of identity, and have sizes higher than those described in other dipterous species. Overall, the results indicate that there are no differences between the individuals studied from the Spanish and French populations.
[EN] In this work we perform molecular studies of the species C. stimulator from the family Oestridae (Diptera), which causes myiasis in roe deer. We have amplified by PCR, cloned and sequenced the molecular markers COI, 28s and ITS-1 and ITS-2 in several individuals from French and Spanish populations. We described for the first time the molecular markers 28S, ITS-1 and ITS-2, and expand the data about COI of the species C. stimulator. We have shown that these markers present very high percentages of identity. Although, they present variations due to base changes, deletions and insertions mutations, sometimes related to microsatellites. ITS-1 and the ITS-2 are the markers that have greater ranges of variation in the percentages of identity, and have sizes higher than those described in other dipterous species. Overall, the results indicate that there are no differences between the individuals studied from the Spanish and French populations.