Identificación molecular de especies de la subfamilia Oestrinae (Díptera, Oestridae)
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2016-03-04
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Jaén: Universidad de Jaén
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[ES] Las miasis son una patología producida como consecuencia de la parasitación por especies de dípteros de la familia Oestridae. Nos centramos en el estudio morfológico y molecular de especies de los géneros Cephenemyia, Pharyngomyia y Oestrus parásitas de las cavidades nasofaringeas. Hemos eestudiado los perítremos anteriores de las especies que pueden constituir un carácter morfológico adicional para diferenciar entre las especies estos dos géneros.
Hemos intentado poner a punto un protocolo de identificación de las diferentes especies mediante PCR específicas del gen de la COI mitocondrial. A pesar de haber diseñado los primeros en las regiones más variables de este gen, los resultados de las PCR no permiten diferenciar las especies.
Sin embargo, los resultados mediante la PCR-RFLP sugieren que mediante esta técnica será factible identificar y diferenciar las distintas especies.
Finalmente los análisis morfológico y moleculares de las larvas LII encontradas en el cerebro y meninges de los corzos indican que estas pertenecen a la especie C. stimulator, a pesar de localizarse fuera de las regiones habituales.
[EN] Myiasis is a pathological process in vertebrates due to parasitism by species of flies of the family Oestridae. We focus on the morphological and molecular study of the species belonging to the Cephenemyia, Pharyngomyia and Oestrus that parasites the nasopharyngeal cavities. We have studied the anterior peritremes of Cephenemyia and Pharyngomyia species, demonstrating that have differential characteristics so that they may constitute an additional morphological character in order to differentiate between the species of both genera. We have tried to identify the different species though the COI gene by PCR. Despite the fact that the primers were designed in the most variable regions of this gen the PCR results do not differentiate the species. However, the results with PCR-RFLP suggested that this technique is feasible to identify and differentiate between the species. Finally the morphological and molecular analysis of LII larvae found in the brain and meninges of the roes indicate that these belong to the species C. stimulator, although these larvae were located outside normal the parasite regions.
[EN] Myiasis is a pathological process in vertebrates due to parasitism by species of flies of the family Oestridae. We focus on the morphological and molecular study of the species belonging to the Cephenemyia, Pharyngomyia and Oestrus that parasites the nasopharyngeal cavities. We have studied the anterior peritremes of Cephenemyia and Pharyngomyia species, demonstrating that have differential characteristics so that they may constitute an additional morphological character in order to differentiate between the species of both genera. We have tried to identify the different species though the COI gene by PCR. Despite the fact that the primers were designed in the most variable regions of this gen the PCR results do not differentiate the species. However, the results with PCR-RFLP suggested that this technique is feasible to identify and differentiate between the species. Finally the morphological and molecular analysis of LII larvae found in the brain and meninges of the roes indicate that these belong to the species C. stimulator, although these larvae were located outside normal the parasite regions.