Análisis del papel de los exosomas durante el desarrollo embrionario
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2024-09-05
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[ES] Los exosomas son vesículas extracelulares liberadas por las células al espacio extracelular que transportan cargas en su interior, como lípidos, proteínas, ARNm, microARNs y ADN entre otras moléculas. Esto supone un sistema de comunicación entre células, por lo que tienen la capacidad de regular muchos procesos biológicos. En este trabajo experimental se quiere determinar el papel que tienen los exosomas durante el desarrollo embrionario, un punto clave del desarrollo embrionario está en el desarrollo del corazón, por lo que, por lo que se han usado células epicárdicas (MEC1), esenciales para comprender los mecanismos del desarrollo cardíaco. Las células se someten a diferentes concentraciones del inhibidor de exosomas GW4869 y se realiza un análisis de la viabilidad celular y cuantificación de proteínas para determinar el efecto que esto causa en ellas. Finalmente, se pudo concluir que el desarrollo y viabilidad celular se ven comprometidos cuando se altera la producción de exosomas, y que esta alteración es dependiente de la dosis de GW4869 que se administra.
[EN] Exosomes are extracellular vesicles released by cells into the extracellular space that carry cargo inside, such as lipids, proteins, mRNA, miRNA and DNA, and other molecules. This creates a communication system between cells, giving them the ability to regulate many biological processes. This experimental study aims to determine the role of exosomes during embryonic development, a key point in embryonic development is in the development of the heart, therefore, epicardial cells from the MEC1 cell line, which are essential for understanding the mechanisms of cardiac development, have been used for this study. The cells are subjected to different concentrations of the exosome inhibitor GW4869 and a cell viability analysis is conducted to determine the effect this has on them. Finally, it could be concluded that cell development and viability were compromised when exosome production was altered, and that this alteration was dependent on the dose of GW4869 that was administered.
[EN] Exosomes are extracellular vesicles released by cells into the extracellular space that carry cargo inside, such as lipids, proteins, mRNA, miRNA and DNA, and other molecules. This creates a communication system between cells, giving them the ability to regulate many biological processes. This experimental study aims to determine the role of exosomes during embryonic development, a key point in embryonic development is in the development of the heart, therefore, epicardial cells from the MEC1 cell line, which are essential for understanding the mechanisms of cardiac development, have been used for this study. The cells are subjected to different concentrations of the exosome inhibitor GW4869 and a cell viability analysis is conducted to determine the effect this has on them. Finally, it could be concluded that cell development and viability were compromised when exosome production was altered, and that this alteration was dependent on the dose of GW4869 that was administered.