El control de contaminación en laboratorio de genética forense
Fecha
2024-09-05
Autores
Título de la revista
ISSN de la revista
Título del volumen
Editor
Resumen
[ES] La contaminación de muestras con ADN exógeno es uno de los grandes problemas que se dan en la genética forense a la hora de la creación de perfiles genéticos para la identificación de víctimas de desastres masivos. Esto es debido a la dificultad que existe en la prevención de la contaminación del ADN de las muestras ya que presentan un alto grado de degradación. Es por ello que en este trabajo se ejecutaron diversos procedimientos para determinar si el control de la contaminación se realiza correctamente en el Laboratorio de Identificación Genética de la Universidad de Granada en el proyecto de Memoria Histórica y Democrática. Entre estos métodos se incluyen: la toma de muestras a través de hisopos bucales, extracción automatizada de ADN, amplificación por PCR y análisis de los STRs autosómicos mediante electroforesis capilar, siendo los resultados presentados en forma de electroferogramas.
[EN] Sample contamination with exogenous DNA is one of the biggest problems that arise in forensic genetics when creating genetic profiles for the identification of victims who have been in graves for decades. This is due to the difficulty in preventing contamination of the samples, during their collection or subsequent analysis, since they presnt a high degree of degradation. In this work several procedures were executed to determine if the contamination control is carried out correctly in the minimum sample laboratory of the Genetic Identification Laboratory of the University of Granada where a Project in the analysis of human bones remains from the Spanish Civil War and the subsequent Franco Dictatoship is been investigated. These methods include: sampling through buccal swabs, automated DNA extraction, PCR amplification and analysis of autosomal STRs by capillary electrophoresis with the results being presented in the form of electropherograms.
[EN] Sample contamination with exogenous DNA is one of the biggest problems that arise in forensic genetics when creating genetic profiles for the identification of victims who have been in graves for decades. This is due to the difficulty in preventing contamination of the samples, during their collection or subsequent analysis, since they presnt a high degree of degradation. In this work several procedures were executed to determine if the contamination control is carried out correctly in the minimum sample laboratory of the Genetic Identification Laboratory of the University of Granada where a Project in the analysis of human bones remains from the Spanish Civil War and the subsequent Franco Dictatoship is been investigated. These methods include: sampling through buccal swabs, automated DNA extraction, PCR amplification and analysis of autosomal STRs by capillary electrophoresis with the results being presented in the form of electropherograms.