Caracterización de ADNs satélite en la hormiga Iberoformica subrufa
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2021-07-28
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Jaén: Universidad de Jaén
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[ES] El estudio del ADN satélite, formado por repeticiones en tándem, se encuentra en auge. Durante años sus funciones han sido una incógnita, pero actualmente se asocia a funciones básicas en el genoma eucariota, como componente principal de la heterocromatina. Este Trabajo de Fin de Grado se centra en el análisis e identificación de las familias de ADN satélite presentes en Iberoformica subrufa, especie perteneciente a la familia Formicidae y orden Hymenoptera. Se ha recurrido al uso de herramientas bioinformáticas para la identificación de secuencias repetitivas a partir de datos de secuenciación masiva de genoma. De este modo se han obtenido 14 familias de ADN satélite en Iberoformica subrufa ocupando un 3,8% del genoma total. Tras esta identificación se ha llevado a cabo un análisis comparativo de estas familias de ADN satélite entre diferentes especies de hormiga cuyos genomas están secuenciados y disponibles en las bases de datos. De ellas, 5 familias de ADN satélite se encontraron en otras especies de hormiga con un alto porcentaje de conservación.
[EN] The study of satellite DNA, made up of tandem repeats, is booming. For years its functions have been unknown but currently it is associated with basic functions in the eukaryotic genome, as the main component of heterochromatin. This Final Degree Project focuses on the analysis and identification of the satellite DNA families present in Iberoformica subrufa, a species belonging to the family Formicidae and the order Hymenoptera. The use of bioinformatics tools has been resorted to for the identification of repetitive sequences from massive genome sequencing data. Thus, 14 satellite DNA families have been obtained in Iberoformica subrufa, occupying 3.8% of the total genome. After this identification, a comparative analysis of these satellite DNA families has been carried out between different ant species whose genomes are sequenced and available in the databases. Of these, 5 families of satellite DNA were found in other ant species with a high percentage of conservation.
[EN] The study of satellite DNA, made up of tandem repeats, is booming. For years its functions have been unknown but currently it is associated with basic functions in the eukaryotic genome, as the main component of heterochromatin. This Final Degree Project focuses on the analysis and identification of the satellite DNA families present in Iberoformica subrufa, a species belonging to the family Formicidae and the order Hymenoptera. The use of bioinformatics tools has been resorted to for the identification of repetitive sequences from massive genome sequencing data. Thus, 14 satellite DNA families have been obtained in Iberoformica subrufa, occupying 3.8% of the total genome. After this identification, a comparative analysis of these satellite DNA families has been carried out between different ant species whose genomes are sequenced and available in the databases. Of these, 5 families of satellite DNA were found in other ant species with a high percentage of conservation.