Aislamiento y actividad antimicrobiana de diferentes cepas de Tichoderma SP. y Gliocladium SP. sobre semillas de Capsicum Annuum, Lactuca Sativa, Solanum Lycopersicum y Raphnus Sativus y sobre sus principales fitopatógenos
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2024-01-09
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Jaén: Universidad de Jaén
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Trichoderma sp. y Gliocladium sp. son hongos que constituyen parte de la microbiota natural de la mayoría de los suelos y es conocido ampliamente por su efecto antagónico contra una gran cantidad de microorganismos fitopatógenos. Se aislaron un total de 27 cepas de Trichoderma sp. y Gliocladium sp. de 11 suelos distintos procedentes de Jaén y Ciudad Real. Utilizando tres tipos de medios para evaluar el mejor método de aislamiento. Los aislados se caracterizaron y se obtuvieron un total de 18 cepas del género Trichoderma sp. y 11 pertenecientes al género Gliocladium sp. Se evaluó con cada uno de los aislados el efecto inhibitorio sobre los hongos Phytophthora sp., Colletotrichum acutatum y Verticillium dahliae. Además de comprobar el efecto sobre semillas de plantas destinadas al consumo humano como: Lechuga (Lactuca sativa), Rabanillo (Raphnus sativus), Tomate cherry (Solanum lycopersicum var. cerasiforme) y Pimiento (Capsicum annuum) y el efecto tras la inoculación de sus patógenos asociados.
[EN] Trichoderma sp. and Gliocladium sp. are fungi that constitute part of the natural microflora of most soils and are widely known for their antagonistic effect against a large number of phytopathogenic microorganisms. A total of 27 strains of Trichoderma sp. and Gliocladium sp. were isolated from 11 different soils from Jaén and Ciudad Real. Using three types of means to evaluate the best method of insulation. The isolates were characterized and a total of 18 strains of the genus of Trichoderma sp. and 11 belonging to the genus of Gliocladium sp. were obtained. The inhibitory effect on Phytophthora sp., Colletotrichum acutatum and Verticillium dahliae was evaluated with each of the isolates. In addition to checking the effect on seeds of plants intended for human consumption such as: Lettuce (Lactuca sativa), Little Radish (Raphnus sativus), Cherry tomato (Solanum lycopersicum var. cerasiforme) and Pepper (Capsicum annuum) and the effect after inoculation of their associated pathogens.
[EN] Trichoderma sp. and Gliocladium sp. are fungi that constitute part of the natural microflora of most soils and are widely known for their antagonistic effect against a large number of phytopathogenic microorganisms. A total of 27 strains of Trichoderma sp. and Gliocladium sp. were isolated from 11 different soils from Jaén and Ciudad Real. Using three types of means to evaluate the best method of insulation. The isolates were characterized and a total of 18 strains of the genus of Trichoderma sp. and 11 belonging to the genus of Gliocladium sp. were obtained. The inhibitory effect on Phytophthora sp., Colletotrichum acutatum and Verticillium dahliae was evaluated with each of the isolates. In addition to checking the effect on seeds of plants intended for human consumption such as: Lettuce (Lactuca sativa), Little Radish (Raphnus sativus), Cherry tomato (Solanum lycopersicum var. cerasiforme) and Pepper (Capsicum annuum) and the effect after inoculation of their associated pathogens.