Caracterización molecular de secuencias repetidas en genomas eucarióticos: Arvicola amphibius
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2024-09-06
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[ES] Este trabajo se centra en analizar y caracterizar las secuencias repetitivas, especialmente los ADN satélites (el satelitoma), del genoma de la especie Arvicola amphibius. El objetivo principal es cuantificar y caracterizar estas secuencias repetidas utilizando datos de bases públicas y programas informáticos como RepeatExplorer y Geneius. El análisis de los datos de secuenciación masiva nos ha permitido demostrar que el satelitoma de A. amphibius está integrado por 18 familias de ADN satélite. En conjunto estas familias representan el 7,861% del genoma de esta especie, mostrando una variación tanto en la abundancia, tamaño de monómero y porcentaje en A+T. El análisis de CHRISMAPP nos ha permitido comprobar que las diferentes familias de ADN satélites se distribuyen por la eucromatina y regiones de heterocomatina de los cromosomas. En general las familias de satDNAs de la especie A. amphibiustienen las características típicas descritas en los satelitomas de otras especies de eucariotas.
[EN] This work focuses in the analysis and characterization of the repetitive sequences, especially the satellite DNA (the satellitome), of the genome of the rodent species Arvicola amphibius. The main objective is to quantify and characterize these repeated sequences using data available in public databases and software such as RepeatExplorer and Geneius. Analysis of the massive sequencing data has shown that the A. amphibius satellitome is composed of 18 satellite DNA families. Together these families represent 7.861% of the genome of this species, showing variation in abundance, monomer size and A+T percentage. CHRISMAPP analysis has allowed us to verify that the different satellite DNA families are distributed throughout the euchromatin and heterocomatin regions of the chromosomes. In general, the satDNA families of the A. amphibius species have the typical characteristics described in the satellitomes of other eukaryote species.
[EN] This work focuses in the analysis and characterization of the repetitive sequences, especially the satellite DNA (the satellitome), of the genome of the rodent species Arvicola amphibius. The main objective is to quantify and characterize these repeated sequences using data available in public databases and software such as RepeatExplorer and Geneius. Analysis of the massive sequencing data has shown that the A. amphibius satellitome is composed of 18 satellite DNA families. Together these families represent 7.861% of the genome of this species, showing variation in abundance, monomer size and A+T percentage. CHRISMAPP analysis has allowed us to verify that the different satellite DNA families are distributed throughout the euchromatin and heterocomatin regions of the chromosomes. In general, the satDNA families of the A. amphibius species have the typical characteristics described in the satellitomes of other eukaryote species.