Caracterización comparativa de tres cepas de enterococos
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2016-07-07
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Jaén: Universidad de Jaén
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[ES]Una dieta alta en grasa es propensa a condicionar las poblaciones microbianas en el intestino. Esto ha sido confirmado por estudios que utilizan dietas altas en grasas/ricas en calorías, en ratones. Este trabajo es parte de un estudio más amplio sobre el efecto específico del aceite de oliva en las poblaciones de bacterias intestinales. Alimentamos ratones con tres dietas altas en grasa (aceite de oliva virgen y mantequilla) y con una dieta estándar. Después del tratamiento, se estudio la población simbiótica en la heces y los intestinos y se aisló una colección de cepas. Varias de estas cepas fueron enterococos y algunos de ellos mostraron ser especialmente interesantes. Aquí presentamos una caracterización proteómica preliminar de tres de ellos y la comparación de los dos más similares, que resultaron pertenecer a la especie E. faecalis.
[EN]A high fat diet is prone to condition the microbial populations in the intestine. This has been confirmed by studies using high fat/high calorie diets on mice. This work is part of a wider study on the specific effect of olive oil on the gut bacterial populations. We fed mice with three high fat diets (virgin and refined olive oil and butter) and with a standard chow diet. After treatment, symbiotic population in faeces and intestines was studied and a collection of strains was isolated. Several of these strains were enterococci and some of them showed to be especially interesting. Here we show a preliminary proteomic characterization of three of them and the comparison of the two most similar ones, which turned out to belong to the E. faecalis species.
[EN]A high fat diet is prone to condition the microbial populations in the intestine. This has been confirmed by studies using high fat/high calorie diets on mice. This work is part of a wider study on the specific effect of olive oil on the gut bacterial populations. We fed mice with three high fat diets (virgin and refined olive oil and butter) and with a standard chow diet. After treatment, symbiotic population in faeces and intestines was studied and a collection of strains was isolated. Several of these strains were enterococci and some of them showed to be especially interesting. Here we show a preliminary proteomic characterization of three of them and the comparison of the two most similar ones, which turned out to belong to the E. faecalis species.